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Koumis Philippou. Susanne Salomon. Takayuki Shindo. Mikhail Baloban. Sara Bergonzi. Elena Boikoglou. Burcu Dartan. Humberto Fernandes. London: Routledge. Recipes and food discourse in English — a historical menu. Diemer, Stefan; and Maximiliane Frobenius. When making pie, all ingredients must be chilled.

Including you: Lexical, syntactic and interactive features in online discourse - a synchronic study of food blogs. The return of the prefix? New verb-particle combinations in blogs. Orthographic annotation of Middle English Corpora. Full text here. Spelling variation in Middle English manuscripts: The case for an integrated corpus approach.

Amsterdam: Benjamins, Corpus Linguistics with Google? It's all a bit upmessing. Non-standard verb-particle combinations in blogs. Das Internet als Korpus? Aktuelle Fragen und Methoden der Korpuslinguistik. The polysemy of over in Late Middle English verb-particle combinations.

Zur historischen Entwicklung der englischen Rechtschreibung. Campus Magazine. Author: Claudia Ehrlich. Endlich verstehen: Geschmackssache - Warum machen wir beim Essen "hmmm" oder ihhh"?. Interview: Martina Kix. SR2 KulturRadio. Interview: Stefan Kolodziej. Wie produziert man Quality-TV? Opus-Kulturmagazin Interview: Claudia Ehrlich.

Rosamunde Pilcher zum Interview: Jochen Marmit. Intercultural Communication through English as a Lingua Franca - Strategies for intercultural awareness and English language teaching. Understanding intercultural communication: Negotiating meaning and identity in English as a Lingua Franca Skype conversations.

Embodied stancetaking in conversation. Master theses Hans, Samira. Environmental Economics and Business Management. Kurtenacker, Rebecca. Case Study: Zero Waste Philosophie. Nadig, Magali. Social Media Marketing. NGOs on social media platforms. The language of social media advertising - A corpus-based analysis of lexical categories and semantic fields on Target's Instagram page. English Linguistics. Clodo, Laura. Umwelt- und Betriebswirtschaft.

Hochschule Trier. Emotion und Engagement bei Nachrichten in den sozialen Medien. Sprache und Kommunikation. Zimmer, Fabian. Social Media Marketing in der Automotive-Branche. KG Development of a comprehensive social media strategy for a medium-sized enterprise. Trier University of Applied Sciences. The discourse of emotion in American television: A case study. Clarenbach, Sarah. Individual variation on Twitter: A corpus-based study of naturalistic data.

Kovaleva, Inna. Burghardt, Sina. Realisation of laughter on Twitter. Malinowski, Sebastian. A linguistic analysis of internet memes. Morschett, Raphael. A linguistic analysis of verbal humour in Annie Hall. Pieczyk, Tessa. The status of the English language in native Ireland — a sociolinguistic case study. Baumann, Janine. Unternehmenskommunikation 2. Buitrago-Barroso, Laura. Morphosyntaktische Integration von Anglizismen im Deutschen durch Sprachkontakt. Hoffmann, Sandra.

A corpus-based study of spelling standardization on Early Modern English. Lackner, Veronika. Schmidt, Selina. Spanglish on Twitter as an expression of Latino identity — a discourse analysis. Maier, Katrin. Fake review detection on Amazon — a forensic linguistic analysis. Pirsch, Nadine Dji-Yung. Schaly, Selina. Bauer, Vivien. Gender and political speech: A linguistic analysis of acceptance speeches by US politicians in the midterm elections. Bernard, Nina-Michelle. Recipes in Instastories — a corpus-based discourse analysis.

Fisch, Helen. ELF in the classroom — An applied study of English as a lingua franca communication strategies and their use in English language teaching. Gab, Katharina. Hauser, Lorena. Metaphors used in U. Republican and Democratic political speeches - A corpus-based cognitive analysis.

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Wilbert, Nicole. Errors and mistakes in classroom settings — a corpus-based study. Witt, Steffen. Bachelor theses Adamek, Jessica. Jung, Andreas. Pramanik, Ahana. Sustainable Business and Technology. Schirra, Ann-Kristin. KG auf Instagram.

Media and Computer Science. Tahta, Funda. Chen, Chu-Li. Click me! German persuasive marketing texts and politeness strategies. Kultur und Technik, Kernfach Sprache und Kommunikation. TU Berlin. Giro, Ann-Kristin. Translation Science.

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Such varied findings are difficult to interpret, given that each trial employed subtly different treatments and controls, says Evan Myers, an epidemiologist who specialises in obstetrics at Duke University Medical Center in Durham, North Carolina. One big unknown is the mechanism by which acupuncture might boost fertility.

While acupuncturists claim to channel a form of energy known as Qi , some western fertility specialists speculate that the needles may somehow improve blood flow to the uterus. She remains sceptical of the physiological benefits of acupuncture, but is nevertheless introducing it in her clinic because it is safe, cheap and may have a positive placebo effect by making women less distressed. Trending Latest Video Free. Fish recorded singing dawn chorus on reefs just like birds Bacteria were the real killers in flu pandemic The scientific guide to a better Christmas dinner.

Dan Hooper: What happened at the big bang? Science with Sam explains The health benefits of sunlight: Can vitamin D help beat covid? The search for the origin of life: From panspermia to primordial soup. This TCSE shows potential to bridge the gap between the monolayer studies and in vivo studies.

Besides basic research, this model also allows testing the efficacy of new agents in the aforementioned clinical conditions. We are grateful to Wendy Stevens for comments and discussion. The study was supported by the Prof.

Harry und Rosa Neumann-Stiftung and the Dr. Paul and Cilli Weill Stiftung. National Center for Biotechnology Information , U. Journal List Indian J Dermatol v. Indian J Dermatol. Author information Article notes Copyright and License information Disclaimer.

Address for correspondence: Dr. E-mail: ed. Received Aug; Accepted Aug. This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4. Abstract Background: Organotypic tissue-cultured skin equivalents are used for a broad range of applications either as possible substitute for animal tests or for transplantation in patient-centered care.

Aims: In this study, we implemented melanocytes in a tissue-cultured full-thickness skin equivalent, consisting of epidermis and dermis. Materials and Methods: Pigmented skin equivalents were morphologically characterized, and melanogenesis was evaluated after treatment with kojic acid — a tyrosinase inhibitor and forskolin — a well-known activator of the cyclic adenosine 3,5-monophosphate pathway. Results: The morphological analysis revealed characteristic epidermal stratification with melanocytes located at the basal layer.

Conclusion: The present study demonstrates that the herein-introduced organotypic tissue-cultured skin equivalent is comparable to the normal human skin and its versatility in tests regarding skin pigmentation. Introduction Skin pigmentation is a prominent aspect of the human complexion. Materials and Methods Cell isolation and tissue-cultured skin equivalent generation Normal human fibroblasts, keratinocytes, and melanocytes were isolated from infantile foreskins of donors with varying pigmentation status Fitzpatrick scale II—V.

Evaluation of the pigmentation status Shifts in pigmentation of TCSEs after 14 days in presence of forskolin or kojic acid or without treatment were evaluated by reflectance colorimetric analysis. Open in a separate window. Figure 1. Evaluation of the pigmentation status of the TCSEs The pigmentation status was on the one hand by total melanin extraction and on the other hand spectrophotometrically determined.

Figure 2. Discussion Skin equivalents are popular in cosmetic and pharmaceutical research,[ 5 , 8 , 9 , 10 , 11 , 12 ] but are also used in the treatment of skin defects. Conclusion We showed morphological and physiological homologies of the herein-presented TCSE to normal human skin and the comparability of an invasive and a noninvasive method to quantitate pigmentation. Financial support and sponsorship Nil. Conflicts of interest There are no conflicts of interest. References 1. Skin as a living coloring book: How epithelial cells create patterns of pigmentation.

Pigment Cell Melanoma Res. The mechanism of melanocyte dendrite formation: The impact of differentiating keratinocytes. Pigment Cell Res. Nicolaidou E, Katsambas AD. Pigmentation disorders: Hyperpigmentation and hypopigmentation. Clin Dermatol. Rheinwald JG, Green H. Serial cultivation of strains human epidermal keratinicytes: the formation of keratinizing colonies form single cells. Evaluation of beneficial and adverse effects of glucocorticoids on a newly developed full-thickness skin model.

Toxicol In Vitro. Discrimination between cutaneous pigmentation and erythema: Comparison of the skin colorimeters dermacatch and mexameter. Skin Res Technol. Levels of dopachrome tautomerase in human melanocytes cultured in vitro. Melanoma Res. Cosmetic efficacy claims in vitro using a three-dimensional human skin model. Int J Cosmet Sci. Use of a standardized reconstructed epidermis kit to assess in vitro the tolerance and the efficacy of cosmetics. Epidermal reconstructs: A new tool to study topical and systemic photoprotective molecules.

J Photochem Photobiol B. Identification of novel in vitro test systems for the determination of glucocorticoid receptor ligand-induced skin atrophy. Skin Pharmacol Physiol. Skin equivalents: Skin from reconstructions as models to study skin development and diseases. Br J Dermatol. Grafting of in vitro cultured melanocytes onto laser-ablated lesions in vitiligo. Acta Derm Venereol. Clinical application of a tissue-cultured skin autograft: An alternative for the treatment of non-healing or slowly healing wounds?

Development and characterization of an engraftable tissue-cultured skin autograft: Alternative treatment for severe electrical injuries. Cells Tissues Organs. Penetration of keratinocyte-derived cytokines into basement membrane. J Cell Physiol. Key regulatory role of dermal fibroblasts in pigmentation as demonstrated using a reconstructed skin model: Impact of photo-aging.

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